Dihydrocoumarin, inonhuhwirira, inoshandiswa mukudya, inoshandiswawo secoumarin inotsiva, inoshandiswa sechizoro chinonaka; Blend cream, kokonati, sinamoni inonaka; Inoshandiswawo sekunhuhwirira kwefodya.
Iyo dihydrocoumarin ine chepfu
Dihydrocoumarin haina chepfu. Dihydrocoumarin chigadzirwa chechisikigo chinowanikwa muyero vanilla rhinoceros. Inogadzirirwa nehydrogenation ye coumarin pamberi penickel catalyst pa 160-200 ℃ uye pasi pekumanikidzwa. Inogona zvakare kushandiswa seyakabikwa, hydrolyzed mune alkaline aqueous mhinduro kugadzira o-hydroxyphenylpropionic acid, kupera simba, yakavharwa-loop yakawanikwa.
Kuchengetedza mamiriro
Yakavharwa uye yakasviba, yakachengetwa munzvimbo inotonhorera uye yakaoma, nzvimbo iri mubhodhoro iduku sezvinobvira pasi pezvibvumirano zvekuchengetedza, uye izere ne nitrogen kuchengetedza. Chengetedza muimba yekuchengetera inotonhorera, ine mhepo. Gara kure nemoto, mvura. Inofanirwa kuchengetwa zvakasiyana kubva kune oxidizer, usasanganise kuchengetedza. Yakashongedzerwa nemhando inoenderana uye huwandu hwemidziyo yemoto.
In vitro kudzidza
In vitro enzymatic assay, dihydrocoumarin induced concentration-dependent inhibition yeSIRT1 (IC50 ye208μM). Kuderedzwa kweSIRT1 deacetylase chiitiko kwakacherechedzwa kunyangwe pamadhora emicromolar (85 ± 5.8 uye 73± 13.7% chiitiko pa 1.6μM uye 8μM, zvichiteerana). Microtubule SIRT2 deacetylase yakavharwawo nenzira yakafanana-inotenderera (IC50 ye295μM).
Mushure memaawa makumi maviri nemana ekuratidzwa, dihydrocoumarin (1-5mM) yakawedzera cytotoxicity muTK6 cell mitsara nenzira inoenderana nedosi. Dihydrocoumarin (1-5mM) yakawedzera apoptosis muTK6 masero mitsara nenzira inotsamira muyero pane imwe nguva ye6-awa. A 5mM dose ye dihydrocoumarin yakawedzera apoptosis panguva ye6-awa nguva muTK6 cell line. Mushure menguva ye24-awa yekuratidzwa, dihydrocoumarin (1-5mM) yakawedzera p53 lysine 373 uye 382 acetylation nenzira inoenderana nedosi mumutsara weTK6 cell.
Nguva yekutumira: Nov-01-2024